Shuttle mutagenesis: a method of transposon mutagenesis for Saccharomyces cerevisiae.
نویسندگان
چکیده
منابع مشابه
Shuttle mutagenesis: a method of transposon mutagenesis for Saccharomyces cerevisiae.
We have extended the method of transposon mutagenesis to the eukaryote, Saccharomyces cerevisiae. A bacterial transposon containing a selectable yeast gene can be transposed into a cloned fragment of yeast DNA in Escherichia coli, and the transposon insertion can be returned to the yeast genome by homologous recombination. Initially, the cloned yeast DNA fragment to be mutagenized was transform...
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Plant Research International, P.O. Box 16, 6700 AA Wageningen, The Netherlands (R.G., A.P); Institute of Molecular Plant Sciences Leiden University, The Netherlands (P.B.F.O., J.H.C.H); Centre de Coopération Internationale en Recherche Agronomique pour le Développement, Montpellier, France (C.S., E.G.); John Innes Centre, Norwich, United Kingdom (A.K., P.C.); University of Milan, Italy (L.C.); ...
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ژورنال
عنوان ژورنال: Proceedings of the National Academy of Sciences
سال: 1986
ISSN: 0027-8424,1091-6490
DOI: 10.1073/pnas.83.3.735